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  Section: Biotechnology Methods » Microbiology
 
 
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Oxidase Test

 
     
 
Content
Microbiology
  Introduction
  The Microscopy
  The Bright Field Microscope
  Introduction to the Microscope and Comparison of Sizes and Shapes of Microorganisms
  Cell Size Measurements: Ocular and Stage Micrometers
  Measuring Depth
  Measuring Area
  Cell Count by Hemocytometer or Measuring Volume
  Measurement of Cell Organelles
  Use of Darkfield Illumination
  The Phase Contrast Microscope
  The Inverted Phase Microscope
  Aseptic Technique and Transfer of Microorganisms
  Control of Microorganisms by using Physical Agents
  Control of Microorganisms by using Disinfectants and Antiseptics
  Control of Microorganisms by using Antimicrobial Chemotherapy
  Isolation of Pure Cultures from a Mixed Population
  Bacterial Staining
  Direct Stain and Indirect Stain
  Gram Stain and Capsule Stain
  Endospore Staining and Bacterial Motility
  Enumeration of Microorganisms
  Biochemical Test for Identification of Bacteria
  Triple Sugar Iron Test
  Starch Hydrolysis Test (II Method)
  Gelatin Hydrolysis Test
  Catalase Test
  Oxidase Test
  IMVIC Test
  Extraction of Bacterial DNA
  Medically Significant Gram–Positive Cocci (GPC)
  Protozoans, Fungi, and Animal Parasites
  The Fungi, Part 1–The Yeasts
  Performance Objectives
  The Fungi, Part 2—The Molds
  Viruses: The Bacteriophages
  Serology, Part 1–Direct Serologic Testing
  Serology, Part 2–Indirect Serologic Testing

Aim
To test the oxidase-producing microorganisms.


Principle
The oxidase determines whether microbes can oxidize certain aromatic amines, e.g., paraaminodimethyl alanine to form a colored end product. This oxidation correlates with the cytochrome oxidase activity of some bacteria, including the genera Pseudomonas and Nisseria. A positive test is important in identifying these genera, and also useful for characterizing the Enterobacteria, which are oxidase-negative.


Materials
  • Glassware
  • Sample culture of Pseudomonas, Bacillus, E. coli., Staphylococcus aureus, and Klebsiella
  • Tetramethyl phenyl diamine
  • Dihydrochloride.
Procedure
    Plate Method: Separate agar plates streaked with Pseudomonas, Klebsiella, and Bacillus are taken, and 1% reagent tetra methyl phenyl diamine hydrochloride is directly added to the plates. The reactions were observed.
 
Figure 41 Indole test   Figure 42 Methyl red
     
 
Figure 43 Voges-Proskauer (VP) test   Figure 44 Citrate
     
 
Figure 45 Lactose broth   Figure 46 Mac conkey
     
Figure 47 Urease












 
     
 
 
     




     
 
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