Molecular Biology of Plant Pathways / Metabolic Engineering of Plant Allyl/Propenyl Phenol and Lignin Pathways
Summary
The biotechnological lignin-reduction endeavors thus far reported have, in general,
afforded plants apparently unsuitable for large-scale cultivation, resulting
from compromised vasculature and perhaps higher susceptibility to pathogen
attacks as well. Similarly, lignin represents a challenge in the processing of plant
biomass (e.g., corn, switchgrass, or miscanthus) for biofuel/bioethanol generation
using current fermentation techniques. Therefore, novel approaches must be
developed if the so-called lignin challenge is to be circumvented for these
purposes. Here, we have suggested some new areas of such exploration aiming
at tackling these issues through the application of a newly elucidated metabolic
process sharing the same lignin biochemical precursors, that is, the biosynthesis of
liquid allyl and propenyl phenols. In the past year, the pertinent enzymes, genes,
and specific biochemical precursors in this process have been isolated and
described, thus lending themselves to exploitation through bioengineering.
Further research in these areas will eventually determine the actual (economic)
potential of the allyl and propenyl phenols as biofuels, intermediate chemicals
for polymer synthesis, and/or specialty chemicals. Success in the research
envisaged here could potentially lead to the development of a multibillion dollar
per year industry within the United States alone. The challenge will be to identify
a viable balance between acceptable structural and defensive properties of
cultivated plants and their uses as sources of economically valuable products.