Clinical Specimens from the Intestinal Tract


Diagnostic Microbiology In Action
  Microbiology of the Intestinal Tract
    The Enterobacteriaceae (Enteric Bacilli)
      Identification of Pure Cultures of Enterobacteriaceae from the Normal Intestinal Flora
      Isolation Techniques for Enteric Pathogens
      Identification Techniques for Enteric Pathogens
      Serological Identification of Enteric Organisms
      Techniques to Distinguish Nonfermentative Gram-Negative Bacilli from Enterobacteriaceae
      Rapid Methods for Bacterial Identification
    Clinical Specimens from the Intestinal Tract
      Culturing a Fecal Sample
      Identification of an Unknown Enteric Organism
      Antimicrobial Susceptibility Test of an Enteric Organism

This exercise provides you with an opportunity to apply your knowledge of the Enterobacteriaceae to making a laboratory diagnosis of an intestinal infection. In Experiment 25.1 you will prepare a culture of your own feces and observe the normal intestinal flora on primary isolation plates. In Experiment 25.2 you will be given a pure culture of one of the Enterobacteriaceae as an “unknown” to be identified. Experiment 25.3 is an antimicrobial susceptibility test of your pure unknown culture. Here you should observe the differences in response of gram-negative enteric bacilli, as compared with streptococci and staphylococci studied earlier, to the most clinically useful antimicrobial agents.