After separating the components of the mixture it is necessary to detect them. As chromatography is often used as a quantitative
technique it is essential to be familiar with the following terms:
- Universal detector: this responds to all compounds eluting from the
column, irrespective of their composition.
- Selective/specific detector: this responds to certain elements or functional
groups. This is a useful approach if the components of the mixture are
- Sensitivity: the ratio of detector signal to sample size (or detector response
per amount of sample).
- Minimum detectable level (MDL): the amount of sample in which the
peak height is at least twice the noise height.
- Linear dynamic range: the concentration range of the sample that is
detectable and where the detector response is linear (between the MDL
and detector saturation).